Data Storage and Data Structure

When you record with the 2P setup you will generate data pairs of .smp and .smh files, the former is the data the latter is the header.

Ideally, you follow the name convention for these files (already set the names in Igor when you record):

SMP_M[#1]_[#2]R_[#3a3b]_[#4]

1. Mouse number of that day, typically 1
2. L for left retina or R for right retina
3. Cell layer (a) and number (b), e.g. GCL0
4. Pharmacology if you used any

For example: SMP_M1_LR_GCL0_chirp

When you put your data on the Euler Storage your file structure should typically look like this (comments marked with #). Note that some files will only be created when you preprocess the data in Igor which is typically not done on the same day.

• 20220315 # Date: yyyymmdd
  • 1 # All data for the left retina
    • Raw # Recorded data
      • SMP_M1_LR_GCL0_chirp.smh
      • SMP_M1_LR_GCL0_chirp.smp
      • SMP_M1_LR_GCL0_mb.smh
      • SMP_M1_LR_GCL0_mb.smp
      • SMP_M1_LR_GCL1_chirp.smh
      • SMP_M1_LR_GCL1_chirp.smp
      • ...
    • Pre # This is where the preprocessed files go
      • SMP_M1_LR_GCL0.h5
      • ...
    • 20220315_.ini # Header file for left retina, create in Igor during preprocessing
    • exp_20220315.pxp # Igor experiment saved before closing the ROI window so that you can modify the ROIs still
  • 2 # All data for the right retina, otherwise as in folder 1
    • ...