Ringer Solution

Chemicals

Chemical	2l	4l
NaCl	14.61	29.22
KCl	0.3728	0.7456
MgCL2.6H2O	0.406	0.812
NaH2PO4	0.3	0.6
Glucose	7.2	14.4
NaHCO3	4.369	8.738
CaCl2	0.588	1.176
L-Glut	0.146	0.292

How To - For beginners

1. Always prepare 1,  2 or 4 l
   1. Fill bottle with a little less than that (> 100 ml less)
   2. Put in magnetic stir bar
   3. Put on stirrer
2. Clean scale with brush
3. Clean table with H2O bidest
4. Adjust scale such that it is plane
5. Put chemicals into right order
   1. The respective lists for the ingredients are found next to them
   2. For example, two rows of four
6. Put plastic dish on scale and zero it
7. Clean spoons with H2O bidest
8. Prepare chemical
   1. Take first/next chemical bottle
   2. Fill cup with at least as much as you need
      1. Never put anything back
      2. Don’t touch anything with the spoon except the chemical
   3. Close chemical lid
   4. Remove excess from cup and but it into waste glass
      1. Don’t ever touch the waste glass with the spoon
   5. Fill chemical into glass
   6. Put chemical bottle back
   7. Clean spoon with paper towel
9. Repeat 8 for next chemical until chemical 6
10. Wait 5 min, while you set stirring level relatively high
11. Continue 8 with the last two chemicals
12. Get every rest into the solution
    1. Clean cup with H2O bidet that goes into the bottle
    2. Clean top of bottle with more H2O bidest to have everything in the solution
13. Stop stirring
14. Fill to the exact level you need
15. Continue stirring
16. Done